| 產品編號 | bs-0197R |
| 英文名稱 | VIPR2 Rabbit pAb |
| 中文名稱 | 腺苷酸環化酶激活肽受體-II/血管活性腸肽受體-II抗體 |
| 別 名 | VIP receptor II; VIP receptor2; VIP receptor-2; PACAP type-3; VIP type-2 receptor; Vip2; VPAC2; VPAC2R; Vasoactive intestinal polypeptide receptor 2; VIP-R-2; Pituitary adenylate cyclase-activating polypeptide type III receptor; PACAP type III receptor; PACAP-R-3; PACAP-R3; VIPR2_HUMAN. VIP-R2; VIP-2R |
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Specific References (3) | bs-0197R has been referenced in 3 publications.
[IF=3.188] Zeqi Tang. et al. Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (Ondatra zibethicus). EUR J HISTOCHEM. 2022 Mar 24; 66(2): 3398 IHC ; Rat.
[IF=2.454] Ye D et al. Spatiotemporal Expression Changes of PACAP and Its Receptors in Retinal Ganglion Cells After Optic Nerve Crush.(2018)J Mol Neurosci. Nov 10. WB&IF ; Rat.
[IF=2.22] Ruan, Ming, et al. "Attenuation of stress-induced gastrointestinal motility disorder by gentiopicroside, from Gentiana macrophylla Pall." Fitoterapia(2015). WB ; Rat.
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| 研究領域 | 細胞生物 免疫學 神經生物學 信號轉導 生長因子和激素 細胞膜受體 G蛋白偶聯受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat VIP receptor II: 81-170/437 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide belonging to the vasoactive intestinal polypeptide/glucagon/ secretin family. It is widely distributed in the body, and a variety of biological actions have been reported. Recent studies have shown that there is a family of PACAP receptors (PACAPRs), and two members of this family have been identified. Mouse PACAPR-3 is a protein of 437 amino acids that has 50% and 51% identity with rat PACAP type I and type II receptors, respectively. It binds to vasoactive intestinal polypeptide as well as PACAP-38 and -27, with a slightly higher affinity for PACAP-38, PACAPR-3 mRNA is expressed at high levels in MIN6, at moderate levels in pancreatic islets and other insulin-secreting cell lines, HIT-T15 and RINm5F, as well as in the lung, brain, stomach, and colon, and at low levels in the heart. PACAPR-3 participates in the regulation of insulin secretion. insulin secretion from MIN6 cells is significantly stimulated by PACAP-38. Function: This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78. Similarity: Belongs to the G-protein coupled receptor 2 family. Database links: Entrez Gene: 7434 Human Omim: 601970 Human SwissProt: P41587 Human Unigene: 585052 Human PACAP 受體分為兩個亞型，PACAP-2型受體對PACAP是特異性的，與PACAP的親和力比VIP高100-1000倍，PACAP-II型受體與PACAP和VIP的親和力相似，對二者無選擇性。此抗體即為后者。 |
| 產品圖片 |
Sample: A431 Cell Lysate at 40 ug
Primary: Anti- VPAC2R (bs-0197R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 63 kD
Sample:
Lane 1: Recombinant human VIPR2 protein, N-Trx-His(bs-42334P)
Primary: Anti-VIPR2 (bs-0197R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 34 kDa
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VIP receptor 2/VPAC2 Polyclonal Antibody, Unconjugated(bs-0197R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-VPAC2R antibody (bs-0197R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 2μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
