| 產品編號 | bs-19567R |
| 英文名稱 | OIT3 Rabbit pAb |
| 中文名稱 | 癌蛋白誘導轉錄因子3抗體 |
| 別 名 | Liver specific zona pellucida domain containing protein; Liver specific ZP domain containing protein; LZP; OIT3_HUMAN; Oncoprotein induced transcript 3. |
| 研究領域 | 腫瘤 發育生物學 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Cow) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OIT3: 1-100/545 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
OIT3 contains one EGF-like domain and one ZP domain. It may be involved in hepatocellular function and development. OIT3 is liver-specific and is expressed only in the hepatocytes; down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines. There are two named isoforms. Function: May be involved in hepatocellular function and development. Subcellular Location: Nucleus envelope. Can be secreted into blood. Tissue Specificity: Liver-specific. Expressed only in the hepatocytes. Down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines. Similarity: Contains 1 EGF-like domain. Contains 1 ZP domain. SWISS: Q8WWZ8 Gene ID: 170392 Database links: Entrez Gene: 170392 Human Entrez Gene: 18302 Mouse Omim: 609330 Human SwissProt: Q8WWZ8 Human SwissProt: Q8R4V5 Mouse Unigene: 8366 Human |
| 產品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-OIT3 (bs-19567R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (OIT3) Polyclonal Antibody, Unconjugated (bs-19567R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (OIT3) Polyclonal Antibody, Unconjugated (bs-19567R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
