| 產品編號 | bs-21049R |
| 英文名稱 | POU6F1/BRN5 Rabbit pAb |
| 中文名稱 | 腦特異性同源POU結構域蛋白5抗體 |
| 別 名 | Brain 5; Brain specific homeobox/POU domain protein 5; Brn 5; Brn 5 protein; BRN5; Emb; MPOU; mPOU homeobox protein; POU class 6 homeobox 1; POU domain class 6 transcription factor 1; TCFB1. |
| 研究領域 | 細胞生物 發育生物學 神經生物學 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human POU6F: 25-120/301 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The POU6F1 gene encodes a protein that is part of a family of transcription factors which exhibit distinct temporal and spatial patterns of expression. Function: Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'). Subcellular Location: Nuclear. Tissue Specificity: In the embryo, expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Similarity: Belongs to the POU transcription factor family. Class-6 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: Q14863 Gene ID: 5463 Database links: Entrez Gene: 5463 Human Entrez Gene: 19009 Mouse Entrez Gene: 30334 Zebrafish SwissProt: Q14863 Human SwissProt: Q07916 Mouse SwissProt: P31367 Zebrafish Unigene: 555886 Human Unigene: 28825 Mouse Unigene: 44845 Rat Unigene: 75078 Zebrafish |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (POU6F1) Polyclonal Antibody, Unconjugated (bs-21049R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
