| 產品編號 | bs-41190R |
| 英文名稱 | Streptolysin O Rabbit pAb |
| 中文名稱 | 鏈球菌溶血素O抗體 |
| 別 名 | SLO; Thiol-activated cytolysin; SPs0132 |
| 研究領域 | 心血管 細胞生物 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Streptococcus |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant Streptolysin O |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Streptolysin is a streptococcal hemolytic exotoxin. Types include streptolysin O (SLO), which is oxygen-labile, and streptolysin S (SLS), which is oxygen-stable. An antibody, antistreptolysin O, can be detected in an antistreptolysin O titre. Streptolysin O is hemolytically active only in a reversibly reduced state, unlike streptolysin S, which is stable in the presence of oxygen. Another difference is that SLO is antigenic, while SLS is not antigenic due to its small size. The main function of streptolysin O is to cause hemolysis (the breaking open of red blood cells) — in particular, beta-hemolysis. Increased levels of aso titre in the blood could cause damage to the heart and joints. In most cases, penicillin is used to treat patients with increased levels of aso titre. Function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation (By similarity). Subunit: Homooligomeric pore complex; when inserted in the host membrane. Subcellular Location: Secreted; Host cell membrane. Similarity: Belongs to the thiol-activated cytolysin family. |
| 產品圖片 |
Sample:
Lane 1: Recombinant Streptolysin O protein, His
Primary: Anti-Streptolysin O (bs-41190R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 62 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
